More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CCL18 recombinant protein (Position: A21-A89). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCL18 |
Protein | C-C motif chemokine 18 |
Uniprot ID | P55774 |
Function | Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. |
Tissue Specificity | Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine beta (chemokine CC) family. |
Aliases | Alternative macrophage activation associated CC chemokine 1 antibody|Alternative macrophage activation-associated CC chemokine 1 antibody|AMAC-1 antibody|AMAC1 antibody|CC chemokine PARC antibody|CCL18 antibody|CCL18(4-69) antibody|CCL18_HUMAN antibody|CKb7 antibody|DC-CK1 antibody|DCCK1 antibody|Dendritic cell chemokine 1 antibody|Macrophage inflammatory protein 4 antibody|MIP-4 antibody|MIP4 antibody|PARC antibody|Pulmonary and activation regulated chemokine antibody|Pulmonary and activation-regulated chemokine antibody|SCYA18 antibody|Small inducible cytokine A18 antibody|Small-inducible cytokine A18 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CCL18 antibody, ASA-B0326--1.jpg
All lanes: Anti-CCL18(ASA-B0326) at 0.5ug/ml
WB: Recombinant Human CCL18 Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti-CCL18(ASA-B0326) at 0.5ug/ml
WB: Recombinant Human CCL18 Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
