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CBL Antibody

ASA-B0323

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
Cbl proto-oncogene, E3 ubiquitin protein ligase Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

E.coli-derived human CBL recombinant protein (Position: A556-T906). Human CBL shares 84% amino acid (aa) sequence identity with mouse CBL.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CBL

Protein

 E3 ubiquitin-protein ligase CBL

Uniprot ID

 P22681

Function

 Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

Tissue Specificity

Sub-cellular localization

 Cytoplasm. Cell membrane. Note: Colocalizes with FGFR2 in lipid rafts at the cell membrane.

Sequence Similarities

 Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.

Aliases

 4732447J05Rik antibody|C CBL antibody|Cas Br M (murine) ecotropic retroviral transforming sequence antibody|Cas-Br-M (murine) ecotropic retroviral transforming sequence antibody|Casitas B lineage lymphoma proto oncogene antibody|Casitas B-lineage lymphoma proto-oncogene antibody|CBL 2 antibody|cbl antibody|CBL_HUMAN antibody|CBL2 antibody|E3 ubiquitin protein ligase CBL antibody|E3 ubiquitin-protein ligase CBL antibody|Oncogene CBL2 antibody|Proto oncogene c CBL antibody|Proto-oncogene c-CBL antibody|RGD1561386 antibody|RING finger protein 55 antibody|RNF55 antibody|Signal transduction protein CBL antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CBL antibody, ASA-B0323--1.jpg
All lanes: Anti-CBL(ASA-B0323) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HepG2 Whole Cell Lysate at 40ug
Lane 4: Colo320 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: Skov Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Predicted b

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