More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calcium channel, voltage-dependent, L type, alpha 1D subunit | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CaV1.3 recombinant protein (Position: M1-I180). Human CaV1.3 shares 99% and 98%amino acid (aa) sequence identity with mouse and rat CaV1.3, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CACNA1D |
Protein | Voltage-dependent L-type calcium channel subunit alpha-1D |
Uniprot ID | Q01668 |
Function | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). |
Tissue Specificity | Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle. |
Sub-cellular localization | Membrane. |
Sequence Similarities | |
Aliases | alpha-1 polypeptide antibody|CAC1D_HUMAN antibody|CACH3 antibody|CACN4 antibody|CACNA 1D antibody|CACNA1D antibody|CACNL1A2 antibody|Calcium channel antibody|Calcium channel L type alpha 1 polypeptide isoform 2 antibody|Calcium channel neuroendocrine/brain type alpha 1 subunit antibody|Calcium channel voltage dependent L type alpha 1 antibody|Calcium channel voltage dependent L type alpha 1D subunit antibody|Cav1.3 antibody|CCHL1A2 antibody|L type antibody|PASNA antibody|SANDD antibody|Voltage dependent L type calcium channel subunit alpha 1D antibody|Voltage gated calcium channel alpha 1 subunit antibody|Voltage gated calcium channel alpha subunit Cav1.3 antibody|Voltage gated calcium channel subunit alpha Cav1.3 antibody|Voltage-dependent L-type calcium channel subunit alpha-1D antibody|Voltage-gated calcium channel subunit alpha Cav1.3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CAV1.3 antibody, ASA-B0317, Western blotting
All lanes: Anti CAV1.3 (ASA-B0317) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 245KD
Observed bind size: 245KD
All lanes: Anti CAV1.3 (ASA-B0317) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 245KD
Observed bind size: 245KD
