More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cathepsin B | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTSB |
Protein | Cathepsin B |
Uniprot ID | P07858 |
Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. |
Tissue Specificity | |
Sub-cellular localization | Lysosome. Melanosome. Secreted, extracellular space . Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Sequence Similarities | Belongs to the peptidase C1 family. |
Aliases | Amyloid precursor protein secretase antibody|APP secretase antibody|APPS antibody|CATB_HUMAN antibody|Cathepsin B antibody|Cathepsin B heavy chain antibody|Cathepsin B1 antibody|CathepsinB antibody|CPSB antibody|CTSB antibody|Cysteine protease antibody|OTTHUMP00000116009 antibody|OTTHUMP00000229510 antibody|OTTHUMP00000229511 antibody|OTTHUMP00000229512 antibody|OTTHUMP00000229514 antibody|OTTHUMP00000229515 antibody|OTTHUMP00000229516 antibody|Preprocathepsin B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cathepsin B antibody, ASA-B0310--1.jpg
All lanes: Anti-Cathepsin B(ASA-B0310) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti-Cathepsin B(ASA-B0310) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
