Caspase-1(P20) Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
caspase 1, apoptosis-related cysteine peptidase	Polyclonal	IgG	Rabbit	Mouse, Rat	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1(137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CASP1
Protein	Caspase-1
Uniprot ID	P29452
Function	Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Tissue Specificity	High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the peptidase C14A family.
Aliases	CASP 1 antibody|CASP-1 antibody|CASP1 antibody|Caspase 1 apoptosis related cysteine peptidase antibody|Caspase 1 apoptosis related cysteine protease antibody|Caspase-1 subunit p20 antibody|Caspase1 antibody|ICE antibody|IL 1 beta converting enzyme antibody|IL 1BC antibody|IL-1 beta-converting enzyme antibody|IL-1BC antibody|IL1 beta converting enzyme antibody|IL1B convertase antibody|IL1BC antibody|IL1BCE antibody|Interleukin 1 beta convertase antibody|Interleukin 1 beta convertase precursor antibody|Interleukin 1 beta converting enzyme antibody|Interleukin 1B converting enzyme antibody|Interleukin-1 beta convertase antibody|Interleukin-1 beta-converting enzyme antibody|P45 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse, Rat	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information