More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| caspase 1, apoptosis-related cysteine peptidase | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1(137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CASP1 |
Protein | Caspase-1 |
Uniprot ID | P29452 |
Function | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. |
Tissue Specificity | High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the peptidase C14A family. |
Aliases | CASP 1 antibody|CASP-1 antibody|CASP1 antibody|Caspase 1 apoptosis related cysteine peptidase antibody|Caspase 1 apoptosis related cysteine protease antibody|Caspase-1 subunit p20 antibody|Caspase1 antibody|ICE antibody|IL 1 beta converting enzyme antibody|IL 1BC antibody|IL-1 beta-converting enzyme antibody|IL-1BC antibody|IL1 beta converting enzyme antibody|IL1B convertase antibody|IL1BC antibody|IL1BCE antibody|Interleukin 1 beta convertase antibody|Interleukin 1 beta convertase precursor antibody|Interleukin 1 beta converting enzyme antibody|Interleukin 1B converting enzyme antibody|Interleukin-1 beta convertase antibody|Interleukin-1 beta-converting enzyme antibody|P45 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
-antibody-ASA-B0284-1.jpg)
Anti-Caspase-1(P20) antibody, ASA-B0284, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Spleen Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Spleen Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate
-antibody-ASA-B0284-2.jpg)