More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| protein phosphatase 3, catalytic subunit, alpha isozyme | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Calcineurin A (488-521aa DAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PPP3CA |
Protein | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform |
Uniprot ID | Q08209 |
Function | Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane . Cell membrane, sarcolemma . Nucleus . Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. |
Sequence Similarities | Belongs to the PPP phosphatase family. PP-2B subfamily. |
Aliases | Alpha isoform formerly PPP2B antibody|Calcineurin A alpha antibody| Calcineurin A1 antibody|CalcineurinA antibody|Calmodulin dependent calcineurin A subunit alpha isoform antibody|Calmodulin-dependent calcineurin A subunit alpha isoform antibody|CALN antibody|CALNA 1 antibody|CALNA antibody|CALNA1 antibody|CAM PRP catalytic subunit antibody|CAM-PRP catalytic subunit antibody|CCN 1 antibody|CCN1 antibody| CNA 1 antibody|CNA alpha antibody|CNA antibody|CNA1 antibody|FLJ26613 antibody|MGC52248 antibody|PP2BA_HUMAN antibody|PPP2B antibody| PPP3CA antibody|Protein phosphatase 2B catalytic subunit antibody|Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody|Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody|Protein phosphatase 3 catalytic subunit alpha isozyme antibody|Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody| Serine/ threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Calcineurin A antibody, ASA-B0264, Western blotting
All lanes: Anti Calcineurin A (ASA-B0264) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 84KD
All lanes: Anti Calcineurin A (ASA-B0264) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 84KD
