More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calcium channel, voltage-dependent, L type, alpha 1F subunit | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CACNA1F recombinant protein (Position: Q1843-L1977). Human CACNA1F shares 87.4% amino acid (aa) sequence identity with mouse CACNA1F. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CACNA1F |
Protein | Voltage-dependent L-type calcium channel subunit alpha-1F |
Uniprot ID | O60840 |
Function | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). |
Tissue Specificity | Expression in skeletal muscle and retina. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1F subfamily. |
Aliases | Aland island eye disease (Forsius Eriksson ocular albinism, ocular albinism type 2) antibody|CAC1F_HUMAN antibody|CACNA 1F antibody|CACNAF antibody| CACNAF1 antibody|Calcium channel voltage dependent alpha 1F subunit antibody|Calcium channel voltage dependent L type alpha 1F subunit antibody| Cav1.4 antibody|Cav1.4alpha1 antibody|COD 3 antibody|COD3 antibody|CORDX 3 antibody|CORDX antibody|CORDX3 antibody|CSNB2A antibody|CSNBX 2 antibody|CSNBX2 antibody|JM 8 antibody|JM8 antibody|JMC 8 antibody|JMC8 antibody|Voltage dependent L type calcium channel subunit alpha F antibody| Voltage gated calcium channel alpha 1F subunit antibody|Voltage gated calcium channel subunit alpha Cav1.4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CACNA1F antibody, ASA-B0261, Western blotting
All lanes: Anti CACNA1F (ASA-B0261) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 221KD
Observed bind size: 221KD
All lanes: Anti CACNA1F (ASA-B0261) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 221KD
Observed bind size: 221KD
