More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calcium channel, voltage-dependent, L type, alpha 1C subunit | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CACNA1C recombinant protein (Position: E1933-H2026). Human CACNA1C shares 88.3% amino acid (aa) sequence identity with both mouse and rat CACNA1C. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CACNA1C |
Protein | Voltage-dependent L-type calcium channel subunit alpha-1C |
Uniprot ID | Q13936 |
Function | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. |
Tissue Specificity | Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. Cell membrane . Note: The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. |
Sequence Similarities | Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. |
Aliases | alpha-1 polypeptide antibody|cardiac muscle antibody|isoform 1 antibody|L type antibody|CAC1C_HUMAN antibody|CACH 2 antibody|CACH2 antibody| CACN 2 antibody|CACN2 antibody|CACNA1C antibody|CACNL1A1 antibody| Calcium channel antibody|Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody|Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody|Calcium channel voltage dependent L type alpha 1C subunit antibody|CaV1.2 antibody|CCHL1A1 antibody|DHPR alpha 1 antibody|DHPR alpha 1 subunit antibody|LQT8 antibody|TS antibody|Voltage dependent L type calcium channel alpha 1C subunit antibody|Voltage dependent L type calcium channel subunit alpha 1C antibody|Voltage gated calcium channel alpha subunit Cav1.2 antibody|Voltage gated calcium channel subunit alpha Cav1.2 antibody|Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody|Voltage-dependent L-type calcium channel subunit alpha-1C antibody|Voltage-gated calcium channel subunit alpha Cav1.2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CACNA1C antibody, ASA-B0260, Western blotting
All lanes: Anti CACNA1C (ASA-B0260) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 249KD
Observed bind size: 249KD
All lanes: Anti CACNA1C (ASA-B0260) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 249KD
Observed bind size: 249KD
