More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| calcium channel, voltage-dependent, P/Q type, alpha 1A subunit | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CACNA1A(430-447aa KTDLLNPEEAEDQLADIA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CACNA1A |
Protein | Voltage-dependent P/Q-type calcium channel subunit alpha-1A |
Uniprot ID | O00555 |
Function | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin- IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA). |
Tissue Specificity | Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P- type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1A subfamily. |
Aliases | APCA antibody|BI antibody|Brain calcium channel 1 antibody|Brain calcium channel I antibody|Cach4 antibody|Cacn3 antibody|Cacna1a antibody|Cacnl1a4 antibody|Calcium channel alpha 1A subunit antibody|Calcium channel L type alpha 1 polypeptide antibody|Calcium channel L type alpha-1 polypeptide isoform 4 antibody|Calcium channel voltage dependent, P/Q type alpha 1A subunit antibody|CAV2.1 antibody|EA2 antibody|FHM antibody|HPCA antibody|MHP antibody|MHP1 antibody|RAT brain class A antibody|RBA-I antibody|SCA6 antibody|Voltage-dependent P/Q-type calcium channel alpha-1A subunit antibody|Voltage-gated calcium channel alpha subunit Cav2.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CACNA1A antibody, ASA-B0259, All Western blotting
All lanes: Anti-CACNA1A(ASA-B0259) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 282KD
Observed bind size: 282KD
All lanes: Anti-CACNA1A(ASA-B0259) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 282KD
Observed bind size: 282KD
