More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BUB1 mitotic checkpoint serine/threonine kinase | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BUB1 |
Protein | Mitotic checkpoint serine/threonine-protein kinase BUB1 |
Uniprot ID | O43683 |
Function | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. |
Tissue Specificity | High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. |
Sub-cellular localization | Nucleus. Chromosome, centromere, kinetochore. Note: Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity). |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. |
Aliases | Bub1 antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody|BUB1 mitotic checkpoint serine/threonine kinase antibody|BUB1, S. cerevisiae, homolog of antibody|BUB1_HUMAN antibody|BUB1A antibody|BUB1L antibody|Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody|Budding uninhibited by benzimidazoles 1 homolog antibody|Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody|hBUB1 antibody|Homolog of mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint serine/threonine protein kinase BUB1 antibody|Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody|Mitotic spindle checkpoint kinase antibody|Putative serine/threonine protein kinase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bub1 antibody, ASA-B0245--1.jpg
All lanes: Anti BUB1 (ASA-B0245) at 0.5ug/ml
WB: Recombinant Human BUB1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti BUB1 (ASA-B0245) at 0.5ug/ml
WB: Recombinant Human BUB1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Bub1 antibody, ASA-B0245--2.jpg
All lanes: Anti BUB1 (ASA-B0245) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
All lanes: Anti BUB1 (ASA-B0245) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
