More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2/adenovirus E1B 19kDa interacting protein 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human BNIP3(38-53aa IYNGDMEKILLDAQHE), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BNIP3 |
Protein | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
Uniprot ID | Q12983 |
Function | Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note: Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane. |
Sequence Similarities | Belongs to the NIP3 family. |
Aliases | BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody|BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody|BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody|BNIP 3 antibody|BNIP3 antibody|BNIP3_HUMAN antibody|NIP 3 antibody|NIP3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BNIP3 antibody, ASA-B0236, Western blotting
All lanes: Anti BNIP3 (ASA-B0236) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MM231Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 30KD
All lanes: Anti BNIP3 (ASA-B0236) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MM231Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 30KD
