More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
glucuronidase, beta | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human beta glucuronidase(GUSB)(50-70aa DFSDNRRRGFEEQWYRRPLWE). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GUSB |
Protein | Beta-glucuronidase |
Uniprot ID | P08236 |
Function | Plays an important role in the degradation of dermatan and keratan sulfates. |
Tissue Specificity | |
Sub-cellular localization | Lysosome. |
Sequence Similarities | |
Aliases | Ac2-223 antibody|asd antibody|Beta G1 antibody|Beta glucuronidase antibody|Beta-G1 antibody|Beta-glucuronidase antibody|BG antibody|BGLR antibody|BGLR_HUMAN antibody|FLJ39445 antibody|Glucuronidase beta antibody|Gur antibody|Gus antibody|Gus-r antibody|Gus-s antibody|Gus-t antibody|Gus-u antibody|GUSB antibody|Gut antibody|MPS7 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-beta glucuronidase(GUSB) antibody, ASA-B0226, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human GUSB, 40.9KD(162aa tag+ M1-P290)
Lane 1: Recombinant Human GUSB Protein 10ng
Lane 2: Recombinant Human GUSB Protein 5ng
Anti-beta glucuronidase(GUSB) antibody, ASA-B0226, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: MCF-7 Cell Lysate