More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
BCL2-related protein A1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human Bcl2A1 (1-16aa, MTDCEFGYIYRLAQDY). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCL2A1 |
Protein | Bcl-2-related protein A1 |
Uniprot ID | Q16548 |
Function | Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity). |
Tissue Specificity | Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | ACC 1 antibody|ACC 2 antibody|ACC1 antibody|ACC2 antibody|B2LA1_HUMAN antibody|Bcl 2 related protein A1 antibody|Bcl-2-like protein 5 antibody|Bcl-2-related protein A1 antibody|BCL2 A1 antibody|BCL2 L5 antibody|BCL2 related protein A1 antibody|Bcl2-L-5 antibody|BCL2A1 antibody|BCL2A1 protein antibody|BCL2L5 antibody|BFL 1 antibody|BFL1 antibody|BFL1 protein antibody|GRS antibody|GRS protein antibody|HBPA 1 antibody|HBPA1 antibody|Hematopoietic BCL2 related protein A1 antibody|Hemopoietic specific early response protein antibody|Hemopoietic-specific early response protein antibody|Protein BFL 1 antibody|Protein BFL-1 antibody|Protein GRS antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Bcl2A1 antibody, ASA-B0207, Western blotting
All lanes: Anti Bcl2A1(ASA-B0207) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted band size: 30KD
Observed band size: 30KD