More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
butyrylcholinesterase | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
E.coli-derived mouse BCHE recombinant protein (Position: K263-E571). Mouse BCHE shares 78% amino acid (aa) sequence identity with human BCHE. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCHE |
Protein | Cholinesterase |
Uniprot ID | Q03311 |
Function | Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. |
Tissue Specificity | Present in most cells except erythrocytes. |
Sub-cellular localization | Secreted . |
Sequence Similarities | |
Aliases | Acylcholine acylhydrolase antibody|BCHE antibody|Butyrylcholine esterase antibody|CHE1 antibody|CHE2 antibody|CHLE_HUMAN antibody|Choline esterase II antibody|Cholinesterase (serum) 2 antibody|Cholinesterase 1 antibody|Cholinesterase antibody|E1 antibody| Pseudocholinesterase antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- BCHE antibody, ASA-B0202, Western blotting
Anti BCHE (ASA-B0202) at 0.5ug/ml
HEPA Whole Cell Lysate at 40ug
Predicted band size: 66KD
Observed band size: 66KD