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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BRCA1 associated protein-1(ubiquitin carboxy-terminal hydrolase) | Monoclonal | IgG | Mouse | Human | ICC, WB | Ascites |
Immunogen |
| Recombinant full-length BAP1. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BAP1 |
Protein | Ubiquitin carboxyl-terminal hydrolase BAP1 |
Uniprot ID | Human(Q92560), Mouse(Q99PU7), Rat(D3ZHS6) |
Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor. |
Tissue Specificity | Highly expressed in testis, placenta and ovary. Expressed in breast. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950). |
Sequence Similarities | Belongs to the peptidase C12 family. BAP1 subfamily. |
Aliases | hucep-6|KIAA0272|UCHL2|BRCA1-associated protein 1|Cerebral protein 6 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BAP1 antibody (monoclonal), ASA-B0196, Western blotting
Lane: MCF-7 Cell Lysate
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