More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor (ligand) superfamily, member 13b | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human BAFF recombinant protein (Position: A134-L285). Human BAFF shares 86.1% amino acid (aa) sequence identity with mouse BAFF. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFSF13B |
Protein | Tumor necrosis factor ligand superfamily member 13B |
Uniprot ID | Q9Y275 |
Function | Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. |
Tissue Specificity | Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. |
Sub-cellular localization | Cell membrane; Single-pass type II membrane protein. |
Sequence Similarities | Belongs to the tumor necrosis factor family. |
Aliases | ApoL related ligand TALL 1 antibody|B cell Activating Factor antibody|B cell Activating Factor antibody|B lymphocyte stimulator antibody|B-cell-activating factor antibody|BAFF antibody|BLYS antibody|CD 257 antibody|CD257 antibody|CD257 antigen antibody|delta BAFF antibody|Dendritic cell derived TNF like molecule antibody|Dendritic cell-derived TNF-like molecule antibody| DTL antibody|DTL precursor antibody|PRO738 antibody|soluble form antibody| TALL 1 antibody|TALL-1 antibody|TALL1 antibody|THANK antibody| TN13B_ HUMAN antibody|TNF and APOL related leukocyte expressed ligand 1 antibody|TNF homolog that activates apoptosis antibody|TNF homolog that activates apoptosis NKFB and JNK. antibody| TNF- and APOL-related leukocyte expressed ligand 1 antibody|TNFSF13B antibody|TNFSF20 antibody| Tumor necrosis factor ligand superfamily member 13B antibody|Tumor necrosis factor ligand superfamily member 20 antibody|Tumor necrosis factor like protein ZTNF4 antibody| Tumor necrosis factor superfamily member 13B antibody|UNQ401 antibody|ZTNF4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- BAFF antibody, ASA-B0186, Western blotting
All lanes: Anti BAFF (ASA-B0186) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted band size: 31KD
Observed band size: 31KD
All lanes: Anti BAFF (ASA-B0186) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted band size: 31KD
Observed band size: 31KD