More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| B-Raf proto-oncogene, serine/threonine kinase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BRAF |
Protein | Serine/threonine-protein kinase B-raf |
Uniprot ID | P15056 |
Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
Tissue Specificity | Brain and testis. |
Sub-cellular localization | Nucleus. Cytoplasm. Cell membrane . Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. |
Sequence Similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. |
Aliases | FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|B Raf proto oncogene serine threonine protein kinase antibody|B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody|BRAF 1 antibody|BRaf antibody|BRaf antibody|BRAF_HUMAN antibody|BRAF1 antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|oncogene BRAF1 antibody|p94 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|RAFB1 antibody|RMIL antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti B Raf, ASA-B0184, Western blot
All lanes: Anti B Raf (ASA-B0184) at 0.5ug/ml
Lane 1: Rat Testis Tissue at 50ug
Lane 2: Rat Brain Tissue at 50ug
Lane 3: Mouse Testis Tissue at 50ug
Lane 4: Mouse Brain Tissue at 50ug
Lane 5: Hela Whole Cell Lysate at 40ug
Lane 6: Jurkat Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: K562 Whole Cell Lysate at 40ug
Predicted band size: 84KD
Observed band size: 84KD
Lane 1: Rat Testis Tissue at 50ug
Lane 2: Rat Brain Tissue at 50ug
Lane 3: Mouse Testis Tissue at 50ug
Lane 4: Mouse Brain Tissue at 50ug
Lane 5: Hela Whole Cell Lysate at 40ug
Lane 6: Jurkat Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: K562 Whole Cell Lysate at 40ug
Predicted band size: 84KD
Observed band size: 84KD