More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATPase, Na+/K+ transporting, alpha 1 polypeptide | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1 (7-34aa RDKYEPAAVSEQGDKKGKKGKKDRDMDE), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATP1A1 |
Protein | Sodium/potassium-transporting ATPase subunit alpha-1 |
Uniprot ID | P05023 |
Function | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Sequence Similarities | |
Aliases | A1A1 antibody|AT1A1 antibody|AT1A1_HUMAN antibody|ATP1A1 antibody|Atpa-1 antibody|ATPase Na+/K+ transporting alpha 1 polypeptide antibody|BC010319 antibody|EC 3.6.3.9 antibody| MGC3285 antibody|MGC38419 antibody| MGC51750 antibody|Na K ATPase alpha A catalytic polypeptide antibody|Na K ATPase catalytic subunit alpha A protein antibody|Na(+)/K(+) ATPase 1 antibody| Na(+)/K(+) ATPase alpha-1 subunit antibody|Na+/K+ ATPase alpha 1 subunit antibody|Na+/K+ ATPase 1 antibody|Nkaa1b antibody|Sodium potassium ATPase alpha 1 polypeptide antibody|Sodium pump 1 antibody|Sodium pump subunit alpha-1 antibody|sodium-potassium ATPase catalytic subunit alpha-1 antibody|Sodium/potassium transporting ATPase alpha 1 chain antibody| Sodium/potassium-transporting ATPase subunit alpha-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ATP1A1 antibody, ASA-B0170, Western blotting
All lanes: Anti ATP1A1 (ASA-B0170) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 113KD
Observed band size: 113KD
All lanes: Anti ATP1A1 (ASA-B0170) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 113KD
Observed band size: 113KD