More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ataxia telangiectasia mutated | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human ATM(36-50aa DPETIKHLDRHSDSK), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATM |
Protein | Serine-protein kinase ATM |
Uniprot ID | Q13315 |
Function | Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. |
Tissue Specificity | Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes. |
Sub-cellular localization | Nucleus. Cytoplasmic vesicle. Note: Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. |
Sequence Similarities | Belongs to the PI3/PI4-kinase family. ATM subfamily. |
Aliases | A-T mutated antibody|A-T mutated homolog antibody|AT complementation group A antibody|AT complementation group C antibody|AT complementation group D antibody|AT complementation group E antibody|AT mutated antibody|AT protein antibody|AT1 antibody|ATA antibody|Ataxia telangiectasia gene mutated in human beings antibody|Ataxia telangiectasia mutated antibody|Ataxia telangiectasia mutated gene antibody|Ataxia telangiectasia mutated homolog(human) antibody|Ataxia telangiectasia mutated homolog antibody|ATC antibody|ATD antibody|ATDC antibody|ATE antibody|ATM antibody|ATM_HUMAN antibody|DKFZp781A0353 antibody|Human phosphatidylinositol 3 kinase homolog antibody|MGC74674 antibody|OTTHUMP00000232981 antibody|Serine protein kinase ATM antibody|Serine-protein kinase ATM antibody|Serine/threonine-protein kinase ATM antibody|T cell prolymphocytic leukemia antibody|Tefu antibody|TEL1 antibody|TEL1, telomere maintenance 1, homolog antibody|TELO1 antibody|Telomere fusion protein antibody|TPLL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ATM antibody, ASA-B0168, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Anti-ATM antibody, ASA-B0168, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MCF-7 Cell Lysate