More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| activating transcription factor 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ATF6 (599-614aa, NINENVINGQDYEVMM), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATF6 |
Protein | Cyclic AMP-dependent transcription factor ATF-6 alpha |
Uniprot ID | P18850 |
Function | Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein. |
Sequence Similarities | Belongs to the bZIP family. ATF subfamily. |
Aliases | Activating transcription factor 6 alpha antibody|Activating transcription factor 6 antibody|ATF 6 antibody|ATF6 alpha antibody|ATF6 antibody|ATF6-alpha antibody|ATF6A antibody|ATF6A_HUMAN antibody|cAMP dependent transcription factor ATF 6 alpha antibody|cAMP-dependent transcription factor ATF-6 alpha antibody|Cyclic AMP dependent transcription factor ATF 6 alpha antibody|DKFZp686P2194 antibody|ESTM49 antibody|FLJ21663 antibody|Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody|Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ATF6 antibody, ASA-B0166, Western blotting
Anti ATF6 (ASA-B0166) at 0.5ug/ml
A549 Whole Cell Lysate at 40ug
Predicted band size: 75KD
Observed band size: 90KD
Anti ATF6 (ASA-B0166) at 0.5ug/ml
A549 Whole Cell Lysate at 40ug
Predicted band size: 75KD
Observed band size: 90KD