More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| activating transcription factor 4(tax-responsive enhancer element B67) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4(332-351aa QYLKDLIEEVRKARGKKRVP), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATF4 |
Protein | Cyclic AMP-dependent transcription factor ATF-4(cAMP-dependent transcription factor ATF-4) |
Uniprot ID | P18848 |
Function | Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Co- localizes with NEK6 in the centrosome. |
Sequence Similarities | Belongs to the bZIP family. |
Aliases | Activating Transcription Factor 4 antibody|ATF 4 antibody|ATF4 antibody|ATF4 protein antibody|ATF4_HUMAN antibody|cAMP-dependent transcription factor ATF-4 antibody|cAMP-responsive element-binding protein 2 antibody|CREB 2 antibody|CREB-2 antibody|CREB2 antibody|Cyclic AMP dependent transcription factor ATF 4 antibody|Cyclic AMP response element binding protein 2 antibody|Cyclic AMP-dependent transcription factor ATF-4 antibody|Cyclic AMP-responsive element-binding protein 2 antibody|DNA binding protein TAXREB67 antibody|DNA-binding protein TAXREB67 antibody|Tax Responsive Enhancer Element B67 antibody|Tax-responsive enhancer element-binding protein 67 antibody|TAXREB67 antibody|TXREB antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ATF4 antibody, ASA-B0165, Western blotting
All lanes: Anti ATF4 (ASA-B0165) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted band size: 39KD
Observed band size: 39KD
All lanes: Anti ATF4 (ASA-B0165) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted band size: 39KD
Observed band size: 39KD