More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aquaporin 9 | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 9 (263-278aa VLFIQMHHSNPDPEVK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AQP9 |
Protein | Aquaporin-9 |
Uniprot ID | Q9JJJ3 |
Function | Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane ; Multi-pass membrane protein . |
Sequence Similarities | |
Aliases | AQP 9 antibody|AQP-9 antibody|AQP9 antibody|AQP9_HUMAN antibody|Aquaglyceroporin-9 antibody|Aquaporin-9 antibody|Aquaporin9 antibody|HsT 17287 antibody|HsT17287 antibody|Small solute channel 1 antibody|SSC 1 antibody|SSC1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Aquaporin 9 antibody, ASA-B0146, Western blotting
All lanes: Anti Aquaporin 9 (ASA-B0146) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Predicted band size: 31KD
Observed band size: 31KD
All lanes: Anti Aquaporin 9 (ASA-B0146) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Predicted band size: 31KD
Observed band size: 31KD