More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aquaporin 8 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Aquaporin 8 (1-19aa MSGEIAMCEPEFGNDKARE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AQP8 |
Protein | Aquaporin-8(AQP-8) |
Uniprot ID | O94778 |
Function | Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. |
Tissue Specificity | Expressed only in pancreas and colon. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | AQP 8 antibody|AQP-8 antibody|AQP8 antibody|AQP8_HUMAN antibody|Aquaporin 8 antibody|Aquaporin-8 antibody|Aquaporin8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Aquaporin 8 antibody, ASA-B0145, Western blotting
Anti Aquaporin 8 (ASA-B0145) at 0.5ug/ml
A431 Whole Cell Lysate at 40ug
Predicted band size: 27KD
Observed band size: 27KD
Anti Aquaporin 8 (ASA-B0145) at 0.5ug/ml
A431 Whole Cell Lysate at 40ug
Predicted band size: 27KD
Observed band size: 27KD
Anti-Aquaporin 8 antibody, ASA-B0145, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate