More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| major intrinsic protein of lens fiber | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0(246-263aa NGQPEGTGEPVELKTQAL), identical to the related rat sequence, and different from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MIP |
Protein | Lens fiber major intrinsic protein |
Uniprot ID | P09011 |
Function | Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity). |
Tissue Specificity | Major component of lens fiber gap junctions. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities | |
Aliases | AQP 0 antibody|AQP0 antibody|Aquaporin antibody|Aquaporin0 antibody|Lens fiber major intrinsic protein antibody|LIM 1 antibody|LIM1 antibody|Major intrinsic protein 26kD antibody|Major intrinsic protein of lens fiber antibody|MIP 26 antibody|MIP antibody|MIP26 antibody|MP 26 antibody|MP26 antibody|PALM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MIP antibody, ASA-B0131, Western blotting
All lanes: Anti MIP (ASA-B0131) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted band size: 28KD
Observed band size: 50KD
All lanes: Anti MIP (ASA-B0131) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted band size: 28KD
Observed band size: 50KD