More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| NEDD8 activating enzyme E1 subunit 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human APPBP1 recombinant protein (Position: E181-L534). Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NAE1 |
Protein | NEDD8-activating enzyme E1 regulatory subunit |
Uniprot ID | Q13564 |
Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
Tissue Specificity | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | 59 kDa antibody|A 116A10.1 antibody|Amyloid beta precursor protein binding protein 1 59kD antibody|Amyloid beta precursor protein binding protein 1 59kDa antibody|Amyloid beta precursor protein binding protein 1 antibody|Amyloid beta precursor protein-binding protein 1 antibody|Amyloid protein binding protein 1 antibody|Amyloid protein-binding protein 1 antibody|APP BP1 antibody|APP-BP1 antibody|APPBP 1 antibody|HPP 1 antibody|HPP1 antibody|NAE 1 antibody|NAE1 antibody|NEDD8 activating enzyme E1 regulatory subunit antibody|NEDD8 activating enzyme E1 subunit 1 antibody|NEDD8 activating enzyme E1 subunit antibody|NEDD8-activating enzyme E1 regulatory subunit antibody|Proto oncogene protein 1 antibody|Proto-oncogene protein 1 antibody|Protooncogene protein 1 antibody|ula 1 antibody|ULA1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- APPBP1 antibody, ASA-B0128, Western blotting
Anti APPBP1 (ASA-B0128) at 0.5ug/ml
Recombinant Human APPBP1 Protein 0.5ng
Predicted band size: 42KD
Observed band size: 42KD
Anti APPBP1 (ASA-B0128) at 0.5ug/ml
Recombinant Human APPBP1 Protein 0.5ng
Predicted band size: 42KD
Observed band size: 42KD