More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| apolipoprotein A-I | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Apolipoprotein A I (42-58aa YVDVLKDSGRDYVSQFE), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APOA1 |
Protein | Apolipoprotein A-I(Apo-AI/ApoA-I) |
Uniprot ID | P02647 |
Function | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
Tissue Specificity | Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the apolipoprotein A1/A4/E family. |
Aliases | Apo AI antibody|Apo-AI antibody|ApoA I antibody|ApoA-I antibody|APOA1 antibody|APOA1/APOC3 fusion gene antibody|APOA1_HUMAN antibody|Apolipoprotein A I precursor antibody|Apolipoprotein A-I(1-242) antibody|Apolipoprotein A1 antibody|Apolipoprotein AI antibody|Apolipoprotein of high density lipoprotein antibody|ApolipoproteinAI antibody|Brp14 antibody|Ltw1 antibody|Lvtw1 antibody|MGC117399 antibody|Sep1 antibody|Sep2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Apolipoprotein A I antibody, ASA-B0120, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: HT1080 Cell Lysate