More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| autophagy related 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7 (673-703aa HSFLEDLTGLTLLHQETQAAEIWDMSDDETI), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ATG7 |
Protein | Ubiquitin-like modifier-activating enzyme ATG7 |
Uniprot ID | O95352 |
Function | E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. |
Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. |
Sub-cellular localization | Cytoplasm . Preautophagosomal structure . Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. |
Sequence Similarities | |
Aliases | 1810013K23Rik antibody|Apg 7 antibody|APG7 autophagy 7 like antibody|APG7 autophagy 7-like (S. cerevisiae) antibody|APG7 like antibody|APG7, S. cerevisiae, homolog of antibody|APG7-like antibody|APG7L antibody|ATG 7 antibody|ATG12-activating enzyme E1 ATG7 antibody|atg7 antibody|ATG7 autophagy related 7 homolog (S. cerevisiae) antibody|ATG7 autophagy related 7 homolog antibody| Atg7l antibody|Autophagy 7, S. cerevisiae, homolog of antibody|Autophagy related protein 7 antibody|Autophagy-related 7 (yeast) antibody|Autophagy-related protein 7 antibody|DKFZp434N0735 antibody|GSA 7 antibody|GSA7 antibody|hAGP7 antibody|Ubiquitin activating enzyme E1 like protein antibody|Ubiquitin-activating enzyme E1-like protein antibody|Ubiquitin-like modifier-activating enzyme ATG7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- APG7 antibody, ASA-B0115, Western blotting
All lanes: Anti APG7 (ASA-B0115) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: 293T Whole Cell Lysate at 40ug
Predicted band size: 78KD
Observed band size: 78KD
All lanes: Anti APG7 (ASA-B0115) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: 293T Whole Cell Lysate at 40ug
Predicted band size: 78KD
Observed band size: 78KD