More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TFAP2A |
Protein | Transcription factor AP-2-alpha |
Uniprot ID | P05549 |
Function | Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the AP-2 family. |
Aliases | Activating enhancer binding protein 2 alpha antibody|Activating enhancer-binding protein 2-alpha antibody|Activator protein 2 antibody|AP 2 transcription factor antibody|AP 2alpha antibody|AP-2 antibody|AP-2 transcription factor antibody|AP2 antibody|AP2 Transcription Factor antibody|AP2-alpha antibody|AP2A_HUMAN antibody|AP2TF antibody|BOFS antibody|FLJ51761 antibody|TFAP 2 antibody|TFAP 2A antibody|TFAP2 antibody|TFAP2A antibody|Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody|Transcription factor AP-2-alpha antibody|Transcription factor AP2 alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-AP2 alpha antibody, ASA-B0107, Western blot
Anti AP2A (ASA-B0107) at 0.5ug/ml
Rat Spleen Tissue Lysate at 50ug
Predicted band size: 48KD
Observed band size: 48KD
Anti AP2A (ASA-B0107) at 0.5ug/ml
Rat Spleen Tissue Lysate at 50ug
Predicted band size: 48KD
Observed band size: 48KD