More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| natriuretic peptide A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ANP (124-151aa, SLRRSSCFGGR- MDRIGAQSGLGCNSFRY), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NPPA |
Protein | Natriuretic peptides A |
Uniprot ID | P01160 |
Function | Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the natriuretic peptide family. |
Aliases | ANF antibody|ANF_HUMAN antibody|ANP antibody|ATFB6 antibody|Atrial natriuretic factor antibody|Atrial natriuretic factor precursor antibody|Atrial natriuretic peptide antibody|Atriopeptin antibody|Cardionatrin antibody|CDD ANF antibody|CDD-ANF antibody|CDP antibody|Natriuretic peptide A antibody|Natriuretic Peptide Precursor A antibody|NPPA antibody|PND antibody|Prepronatriodilatin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ANP antibody, ASA-B0105, Western blotting
All lanes: Anti ANP (ASA-B0105) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted band size: 17KD
Observed band size: 17KD
All lanes: Anti ANP (ASA-B0105) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted band size: 17KD
Observed band size: 17KD