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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| angiopoietin-like 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin-like 4 (349-367aa WWFGTCSHSNLNGQYFRSI), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANGPTL4 |
Protein | Angiopoietin-related protein 4 |
Uniprot ID | Q9BY76 |
Function | Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). |
Tissue Specificity | Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 fibrinogen C-terminal domain. |
Aliases | Angiopoietin like 4 antibody|Angiopoietin related protein 4 antibody|Angiopoietin-like protein 4 antibody|Angiopoietin-related protein 4 antibody|ANGL4_HUMAN antibody|ANGPT L2 antibody|ANGPT L4 antibody|ANGPTL2 antibody|ANGPTL4 antibody|ARP4 antibody|Fasting induced adipose factor antibody|FIAF antibody|Hepatic angiopoietin related protein antibody|Hepatic fibrinogen/angiopoietin related protein antibody|Hepatic fibrinogen/angiopoietin-related protein antibody|HFARP antibody|NL2 antibody|Peroxisome proliferator-activated receptor(PPAR) gamma induced angiopoietin related protein antibody|PGAR antibody|pp1158 antibody|PPARG angiopoietin related protein antibody|PSEC0166 antibody|Weakly similar to angiopoietin 1[H.sapiens] antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ANGPTL4 antibody, ASA-B0086, Western blotting
All lanes: Anti ANGPTL4 (ASA-B0086) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 50KD
Observed band size: 50KD
All lanes: Anti ANGPTL4 (ASA-B0086) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted band size: 50KD
Observed band size: 50KD