More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| angiopoietin 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin 2(454-468aa NLNGMYYPQRQNTNK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANGPT2 |
Protein | Angiopoietin-2(ANG-2) |
Uniprot ID | O15123 |
Function | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 fibrinogen C-terminal domain. |
Aliases | AGPT 2 antibody|Agpt2 antibody|ANG 2 antibody|ANG-2 antibody|ANG2 antibody|Angiopoietin 2a antibody|Angiopoietin 2B antibody|Angiopoietin-2 antibody|Angiopoietin2 antibody|ANGP2_HUMAN antibody|ANGPT 2 antibody|ANGPT2 antibody|ANGPT-2 antibody|Tie2 ligand antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Angiopoietin 2 antibody, ASA-B0084, Western blotting
Lane 1: Recombinant Human ANG2 Protein 10ng
Lane 2: Recombinant Human ANG2 Protein 5ng
Lane 3: Recombinant Human ANG2 Protein 2.5ng
Lane 1: Recombinant Human ANG2 Protein 10ng
Lane 2: Recombinant Human ANG2 Protein 5ng
Lane 3: Recombinant Human ANG2 Protein 2.5ng