More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ArfGAP with FG repeats 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1(5-28aa AKRKQEEKHLKMLRDMTGLPHNRK), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AGFG1 |
Protein | Arf-GAP domain and FG repeat-containing protein 1 |
Uniprot ID | P52594 |
Function | Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Nucleus. Cytoplasmic vesicle. |
Sequence Similarities | Contains 1 Arf-GAP domain. |
Aliases | AGFG1 antibody|AGFG1_HUMAN antibody|Arf-GAP domain and FG repeats-containing protein 1 antibody|ArfGAP with FG repeats 1 antibody|AU045498 antibody|C130049H11Rik antibody|C85612 antibody|D730048C23Rik antibody| DKFZp686I15205 antibody|HIV 1 Rev binding protein antibody| HIV-1 Rev-binding protein antibody|HRB antibody|MGC116375 antibody|MGC116938 antibody| MGC116940 antibody|Nucleoporin like protein RIP antibody|Nucleoporin-like protein RIP antibody|RAB antibody|Rev interacting protein antibody|Rev-interacting protein antibody| Rev/Rex activation domain-binding protein antibody|RIP antibody| |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- AGFG1 antibody, ASA-B0049, Western blotting
All lanes: Anti AGFG1 (ASA-B0049) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted band size: 58KD
Observed band size: 58KD
All lanes: Anti AGFG1 (ASA-B0049) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted band size: 58KD
Observed band size: 58KD