More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| adrenoceptor alpha 1A | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ADRA1A recombinant protein (Position: R235-V466). Human ADRA1A shares 89% and 90% amino acid (aa) sequences identity with mouse and rat ADRA1A, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ADRA1A |
Protein | Alpha-1A adrenergic receptor |
Uniprot ID | P35348 |
Function | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
Tissue Specificity | Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. |
Sub-cellular localization | Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. |
Sequence Similarities | |
Aliases | ADA1D_HUMAN antibody|Adra1 antibody|Adra1a antibody|Adra1d antibody|ADRA1R antibody|Adrd1 antibody|Adrenergic alpha1A receptor antibody|Adrenergic alpha1D receptor antibody|Adrenergic receptor alpha 1d antibody|Adrenergic receptor delta1 antibody|Adrenoceptor alpha 1D antibody|Alpha 1D adrenoceptor antibody|Alpha 1D adrenoreceptor antibody|Alpha adrenergic receptor 1a antibody|Alpha-1A adrenergic receptor antibody|Alpha-1D adrenergic receptor antibody|Alpha-1D adrenoceptor antibody|Alpha-1D adrenoreceptor antibody|Alpha-adrenergic receptor 1a antibody|ALPHA1 antibody|Alpha1A adrenergic receptor antibody|Alpha1D adrenergic receptor antibody|Alpha1DAR antibody|DAR antibody|dJ779E11.2 antibody|Gpcr8 antibody|RA42 antibody|Spr8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADRA1A antibody, ASA-B0044, western blotting
All lanes: Anti-ADRA1A(ASA-B0044) at 0.5ug/ml
WB: Recombinant Human ADRA1A Protein 0.5ng
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti-ADRA1A(ASA-B0044) at 0.5ug/ml
WB: Recombinant Human ADRA1A Protein 0.5ng
Predicted bind size: 51KD
Observed bind size: 51KD