More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ADAM metallopeptidase domain 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM2(701-715aa VLIAIMVKVNFQRKK). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ADAM2 |
Protein | Disintegrin and metalloproteinase domain-containing protein 2 |
Uniprot ID | Q99965 |
Function | Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. |
Tissue Specificity | Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 disintegrin domain. |
Aliases | A disintegrin and metalloproteinase domain 2 antibody|ADAM 2 antibody|ADAM metallopeptidase domain 2 antibody|Adam2 antibody|ADAM2_HUMAN antibody|AI323749 antibody|Cancer/testis antigen 15 antibody|CRYN1 antibody|CRYN2 antibody|CT15 antibody|Disintegrin and metalloproteinase domain-containing protein 2 antibody|Fertilin beta antibody|Fertilin subunit beta antibody|Ftnb antibody|PH 30 antibody|PH 30b antibody|PH-30 antibody|PH30 antibody|Ph30 beta antibody|PH30-beta antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAM2 antibody, ASA-B0035, Western blotting
SMMC Cell Lysate
