More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ADAM metallopeptidase domain 17 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17(806-824aa AASFKLQRQNRVDSKETEC), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ADAM17 |
Protein | Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17) |
Uniprot ID | P78536 |
Function | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. |
Tissue Specificity | Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 disintegrin domain. |
Aliases | A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme) antibody|A disintegrin and metalloproteinase domain 17 antibody|ADA17_HUMAN antibody|ADAM 17 antibody|ADAM metallopeptidase domain 17 antibody|ADAM17 antibody|ADAM17 protein antibody|CD 156b antibody|CD156b antibody|CD156b antigen antibody|CSVP antibody|Disintegrin and metalloproteinase domain-containing protein 17 antibody|MGC71942 antibody|Snake venom like protease antibody|Snake venom-like protease antibody|TACE antibody|TNF alpha convertase antibody|TNF alpha converting enzyme antibody|TNF-alpha convertase antibody|TNF-alpha-converting enzyme antibody|Tumor Necrosis Factor Alpha Converting Enzyme antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAM17 antibody, ASA-B0033, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: 293T Cell Lysate
Lane 1: Human Placenta Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: 293T Cell Lysate