More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ADAM metallopeptidase domain 10 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10(612-627aa SVQWSRHFSGRTITLQ). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ADAM10 |
Protein | Disintegrin and metalloproteinase domain-containing protein 10(ADAM 10) |
Uniprot ID | O14672 |
Function | Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala- |
Tissue Specificity | Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Note: Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. |
Sequence Similarities | Contains 1 disintegrin domain. |
Aliases | A disintegrin and metalloprotease domain 10 antibody|A disintegrin and metalloproteinase domain 10 antibody|AD 10 antibody|AD10 antibody|ADA10_HUMAN antibody|ADAM 10 antibody|ADAM metallopeptidase domain 10 antibody|ADAM10 antibody|CD 156c antibody|CD156c antibody|CD156c antigen antibody|CDw156 antibody|disintegrin and metalloproteinase domain containing protein 10 antibody|Disintegrin and metalloproteinase domain-containing protein 10 antibody|HsT 18717 antibody|HsT18717 antibody|Kuz antibody|Kuzbanian antibody|Kuzbanian protein homolog antibody|MADM antibody|Mammalian disintegrin metalloprotease antibody|Mammalian disintegrin-metalloprotease antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAM10 antibody, ASA-B0032, Western blotting
Lane 1: Recombinant Human ADAM10 Protein 10ng
Lane 2: Recombinant Human ADAM10 Protein 5ng
Lane 3: Recombinant Human ADAM10 Protein 2.5ng
Lane 1: Recombinant Human ADAM10 Protein 10ng
Lane 2: Recombinant Human ADAM10 Protein 5ng
Lane 3: Recombinant Human ADAM10 Protein 2.5ng