More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| acetylcholinesterase (Yt blood group) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ACHE (592-614aa SSYMVHWKNQFDHYSKQDRCSDL), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ACHE |
Protein | Acetylcholinesterase |
Uniprot ID | P22303 |
Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. |
Tissue Specificity | Isoform H is highly expressed in erythrocytes. |
Sub-cellular localization | Cell junction, synapse. |
Sequence Similarities | Belongs to the type-B carboxylesterase/lipase family. |
Aliases | ACEE antibody|ACES_HUMAN antibody|Acetylcholine acetylhydrolase antibody| Acetylcholinesterase antibody|Acetylcholinesterase YT blood group antibody| ACHE antibody| ACHE protein antibody|Apoptosis related acetylcholinesterase antibody|ARACHE antibody|N ACHE antibody|N-ACHE antibody|YT antibody|Yt blood group antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ACHE antibody, ASA-B0026, Western blotting
All lanes: Anti ACHE (ASA-B0026) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted band size: 68KD
Observed band size: 68KD
All lanes: Anti ACHE (ASA-B0026) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted band size: 68KD
Observed band size: 68KD