More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2B Receptor (446-478aa MRLRSSTIQSSSIILLDTLLLTENEGDKTEEQ V), different from the related mouse sequence by six amino acids, and from the related rat sequence by nine amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HTR2B |
Protein | 5-hydroxytryptamine receptor 2B |
Uniprot ID | P41595 |
Function | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down- stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5- hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine. |
Tissue Specificity | Ubiquitous. Detected in liver, kidney, heart, pulmonary artery, and intestine. Detected at lower levels in blood, placenta and brain, especially in cerebellum, occipital cortex and frontal cortex. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome . |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | 5 HT 2B antibody|5 HT 2B receptor antibody|5 HT(2B) antibody|5 HT2B antibody|5 hydroxytryptamine (serotonin) receptor 2B antibody|5 hydroxytryptamine (serotonin) receptor 2B G protein coupled antibody|5 hydroxytryptamine 2B receptor antibody|5 hydroxytryptamine receptor 2B antibody|5-HT-2B antibody|5-HT2B antibody|5-hydroxytryptamine receptor 2B antibody|5HT(2B) antibody|5HT2B_HUMAN antibody| 5HT2F antibody|Htr 2b antibody|Htr2b antibody|NP75 protein antibody|SEROTONIN 5HT2B RECEPTOR antibody|Serotonin receptor 2B antibody| Stomach fundus serotonin receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- 5HT2B Receptor antibody, ASA-B0008, Western blotting
All lanes: Anti 5HT2B Receptor (ASA-B0008) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted band size: 54KD
Observed band size: 54KD
All lanes: Anti 5HT2B Receptor (ASA-B0008) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted band size: 54KD
Observed band size: 54KD