More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human KCNN4(14-29aa RRKRLLEQEKSLAGWA), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KCNN4 |
Protein | Intermediate conductance calcium-activated potassium channel protein 4 |
Uniprot ID | O15554 |
Function | Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. |
Tissue Specificity | Widely expressed in non-excitable tissues. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | hIKCa1 antibody|hKCa4 antibody|hSK4 antibody|IK1 antibody|IKCa1 antibody|Intermediate conductance calcium activated potassium channel protein 4 antibody|Intermediate conductance calcium-activated potassium channel protein 4 antibody|KCa3.1 antibody|KCa4 antibody|KCNN 4 antibody|Kcnn4 antibody|KCNN4_HUMAN antibody|Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody|Putative Gardos channel antibody|SK4 antibody|SKCa 4 antibody|SKCa4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-KCNN antibody, ASA-B1111, Western blotting
All lanes: Anti KCNN(ASA-B1111) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti KCNN(ASA-B1111) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
