More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| potassium voltage-gated channel, subfamily H (eag-related), member 2 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human KCNH2 recombinant protein (Position: L856-S1159). Human KCNH2 shares 92% amino acid (aa) sequence identity with both mouse and rat KCNH2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KCNH2 |
Protein | Potassium voltage-gated channel subfamily H member 2 |
Uniprot ID | Q12809 |
Function | Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoforms USO have no channel activity by themself, but modulates channel characteristics by forming heterotetramers with other isoforms which are retained intracellularly and undergo ubiquitin-dependent degradation. |
Tissue Specificity | Highly expressed in heart and brain. Isoforms USO are frequently overexpressed in cancer cells. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.1/KCNH2 sub-subfamily. |
Aliases | eag homolog antibody|Eag-related protein 1 antibody|ERG antibody|ERG-1 antibody|ERG1 antibody|Ether a go go related potassium channel protein antibody|Ether-a-go-go-related gene potassium channel 1 antibody|Ether-a-go-go-related protein 1 antibody|H ERG antibody|H-ERG antibody|HERG 1 antibody|HERG antibody|hERG-1 antibody|HERG1 antibody|KCNH2 antibody|KCNH2_HUMAN antibody|Kv11.1 antibody|LQT 2 antibody|LQT2 antibody|Potassium channel HERG antibody|Potassium voltage gated channel subfamily H (eag related) member 2 antibody|Potassium voltage-gated channel subfamily H member 2 antibody|SQT1 antibody|Voltage gated potassium channel, subfamily H, member 2 antibody|Voltage-gated potassium channel subunit Kv11.1 antibody|Voltagegated potassium channel, subfamily H, member 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- KCNH2 antibody, ASA-B1109, Western blotting
All lanes: Anti KCNH2 (ASA-B1109) at 0.5ug/ml
WB: Recombinant Human KCNH2 Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti KCNH2 (ASA-B1109) at 0.5ug/ml
WB: Recombinant Human KCNH2 Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
Anti- KCNH2 antibody, ASA-B1109, Western blotting
All lanes: Anti KCNH2 (ASA-B1109) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
All lanes: Anti KCNH2 (ASA-B1109) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
