More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kallikrein-related peptidase 10 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 10(252-265aa SAQHPAVYTQICKY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KLK10 |
Protein | Kallikrein-10 |
Uniprot ID | O43240 |
Function | Has a tumor-suppressor role for NES1 in breast and prostate cancer. |
Tissue Specificity | Expressed in breast, ovary and prostate. |
Sub-cellular localization | Secreted . |
Sequence Similarities | Belongs to the peptidase S1 family. Kallikrein subfamily. |
Aliases | breast normal epithelial cell associated serine protease antibody|Kallikrein related peptidase 10 antibody|Kallikrein10 antibody|KLK 10 antibody|KLK10 antibody|NES 1 antibody|NES1 antibody|Normal epithelial cell specific 1 antibody|Protease serine like 1 antibody|PRSS L1 antibody|PRSSL 1 antibody|PRSSL1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kallikrein 10 antibody, ASA-B1093, Western blotting
All lanes: Anti Kallikrein 10 (ASA-B1093) at 0.5ug/ml
Lane 1: Rat Ovary Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Liver Tissue Lysate at 50ug
Lane 5: 22RV Whole Cell Lysate at 40ug
Lane 6: SROV Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: MM231 Whole Cell Lysat
All lanes: Anti Kallikrein 10 (ASA-B1093) at 0.5ug/ml
Lane 1: Rat Ovary Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Liver Tissue Lysate at 50ug
Lane 5: 22RV Whole Cell Lysate at 40ug
Lane 6: SROV Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: MM231 Whole Cell Lysat
