More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interferon regulatory factor 8 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human IRF8(411-426aa ASHQRSFFRENQQITV), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IRF8 |
Protein | Interferon regulatory factor 8 |
Uniprot ID | Q02556 |
Function | Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). |
Tissue Specificity | Predominantly expressed in lymphoid tissues. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the IRF family. |
Aliases | H ICSBP antibody|H-ICSBP antibody|HGNC:5358 antibody|HGNC5358 antibody|HICSBP antibody|ICSBP 1 antibody|ICSBP antibody|ICSBP1 antibody|Interferon Consensus Sequence Binding Protein 1 antibody|Interferon consensus sequence binding protein antibody|Interferon consensus sequence-binding protein antibody|Interferon Regulatory Factor 8 antibody|IRF 8 antibody|IRF-8 antibody|IRF8 antibody|IRF8_HUMAN antibody|MYLS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IRF8 antibody, ASA-B1072, Western blotting
Lane 1: RAJI Cell Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 1: RAJI Cell Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
