More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interferon regulatory factor 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human IRF5(456-472aa EQFKELHHIWQSQQRLQ), identical to the related rat sequence, and different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IRF5 |
Protein | Interferon regulatory factor 5 |
Uniprot ID | Q13568 |
Function | Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. |
Sequence Similarities | Belongs to the IRF family. |
Aliases | Interferon regulatory factor 5 antibody|Interferon regulatory factor 5 bone marrow variant antibody|IRF 5 antibody|IRF-5 antibody|Irf5 antibody|IRF5_HUMAN antibody|SLEB10 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IRF5 antibody, ASA-B1070, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
