More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin, beta 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 4(1810-1822aa TLSTHMDQQFFQT), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITGB4 |
Protein | Integrin beta-4 |
Uniprot ID | P16144 |
Function | Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. |
Tissue Specificity | Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note: Colocalizes with DST at the leading edge of migrating keratinocytes. |
Sequence Similarities | Belongs to the integrin beta chain family. |
Aliases | CD 104 antibody|CD104 antibody|CD104 antigen antibody|GP150 antibody|Integrin beta 4 subunit antibody|Integrin beta-4 antibody|ITB4_HUMAN antibody|ITG B4 antibody|ITGB 4 antibody|ITGB4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ITGB4 antibody, ASA-B1049, Western blotting
All lanes: Anti ITGB4 (ASA-B1049) at 0.5ug/ml
WB: A431 Whole Cell Lysate at 40ug
Predicted bind size: 200KD
Observed bind size: 200KD
All lanes: Anti ITGB4 (ASA-B1049) at 0.5ug/ml
WB: A431 Whole Cell Lysate at 40ug
Predicted bind size: 200KD
Observed bind size: 200KD
