More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 6 | Polyclonal | IgG | Rabbit | Mouse | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived mouse IL6 recombinant protein (Position: F25-T211). Mouse IL6 shares 40% and 85% amino acid (aa) sequences identity with human and rat IL6, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL6 |
Protein | Interleukin-6 |
Uniprot ID | P08505 |
Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Interleukin BSF 2 antibody|B cell differentiation factor antibody|B cell stimulatory factor 2 antibody|B-cell stimulatory factor 2 antibody|BSF 2 antibody|BSF-2 antibody|BSF2 antibody|CDF antibody|CTL differentiation factor antibody|Cytotoxic T cell differentiation factor antibody|Hepatocyte stimulating factor antibody|HGF antibody|HPGF antibody|HSF antibody|Hybridoma growth factor antibody|Hybridoma growth factor Interferon beta-2 antibody|Hybridoma plasmacytoma growth factor antibody|IFN-beta-2 antibody|IFNB2 antibody|IL 6 antibody|IL-6 antibody|IL6 antibody|IL6 protein antibody|IL6_HUMAN antibody|Interferon beta 2 antibody|Interferon beta-2 antibody|Interleukin 6 (interferon beta 2) antibody|Interleukin 6 antibody|Interleukin BSF 2 antibody|Interleukin-6 antibody|Interleukin6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Mouse | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL6 antibody, ASA-B1029--1.jpg
All lanes: Anti-IL6(ASA-B1029) at 0.5ug/ml
Lane 1: HEPA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
All lanes: Anti-IL6(ASA-B1029) at 0.5ug/ml
Lane 1: HEPA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
