More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 5 receptor, alpha | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA(53-68aa NPDQEQRNVNLEYQVK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL5RA |
Protein | Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA) |
Uniprot ID | Q01344 |
Function | This is the receptor for interleukin-5. The alpha chain binds to IL5. |
Tissue Specificity | Expressed on eosinophils and basophils. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the type I cytokine receptor family. Type 5 subfamily. |
Aliases | CD125 antibody|CD125 antigen antibody|CDw125 antibody|HSIL5R3 antibody|IL-5 receptor subunit alpha antibody|IL-5R subunit alpha antibody|IL-5R-alpha antibody|IL-5RA antibody|IL5R antibody|IL5RA antibody|IL5RA_HUMAN antibody|Interleukin 5 Receptor alpha antibody|Interleukin 5 Receptor alpha chain antibody|Interleukin 5 receptor alpha subunit antibody|Interleukin 5 receptor subunit alpha antibody|Interleukin 5 receptor type 3 antibody|Interleukin-5 receptor subunit alpha antibody|MGC26560 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL5RA antibody, ASA-B1026, Western blotting
All lanes: Anti IL5RA (ASA-B1026) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Lane 8: RAJI Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at 40ug
Predicted b
All lanes: Anti IL5RA (ASA-B1026) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Lane 8: RAJI Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at 40ug
Predicted b
