More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 3(colony-stimulating factor, multiple) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human IL3(62-82aa EDQDILMENNLRRPNLEAFNR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL3 |
Protein | Interleukin-3(IL-3) |
Uniprot ID | P08700 |
Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. |
Tissue Specificity | Activated T-cells, mast cells, natural killer cells. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the IL-3 family. |
Aliases | Burst promoting activity antibody|Colony stimulating factor multiple antibody|Eosinophil colony stimulating factor antibody|Hematopoietic growth factor antibody|IL 3 antibody|IL-3 antibody|IL3 antibody|Interleukin 3(colony stimulating factor, multiple) antibody|Interleukin 3 antibody|Interleukin-3 antibody|Mast cell growth factor antibody|MCGF antibody|MGC79398 antibody|MGC79399 antibody|Multi CSF antibody|Multilineage colony stimulating factor antibody|Multipotential colony stimulating factor antibody|Multipotential colony-stimulating factor antibody|OTTHUMP00000065963 antibody|P cell stimulating factor antibody|P-cell-stimulating factor antibody|Thy1 inducing factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL3 antibody, ASA-B1018, Western blotting
Lane 1: Recombinant Human IL-3 Protein 10ng
Lane 2: Recombinant Human IL-3 Protein 5ng
Lane 3: Recombinant Human IL-3 Protein 2.5ng
Lane 1: Recombinant Human IL-3 Protein 10ng
Lane 2: Recombinant Human IL-3 Protein 5ng
Lane 3: Recombinant Human IL-3 Protein 2.5ng
