More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 18(interferon-gamma-inducing factor) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of Human IL18(175-193aa KKEDELGDRSIMFTVQNED). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL18 |
Protein | Interleukin-18 |
Uniprot ID | Q14116 |
Function | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the IL-1 family. |
Aliases | Iboctadekin antibody|IFN gamma inducing factor antibody|IFN-gamma-inducing factor antibody|IGIF antibody|IL 1 gamma antibody|IL 18 antibody|IL 1g antibody|IL-1 gamma antibody|IL-18 antibody|IL1 gamma antibody|IL18 antibody|IL18 protein antibody|IL1F4 antibody|IL1g antibody|IL1gamma antibody|ILIF4 antibody|Interferon gamma inducing factor antibody|Interferon gamma-inducing factor antibody|Interleukin 1 gamma antibody|Interleukin 18 antibody|Interleukin-1 gamma antibody|Interleukin-18 antibody|Interleukin18 antibody|MGC12320 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IL-18 antibody, ASA-B0990, Western blotting
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Lane 4: RAJI Cell Lysate
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Lane 4: RAJI Cell Lysate
