More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| interleukin 15 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human IL15 recombinant protein (Position: N49-S162). Human IL15 shares 70% amino acid (aa) sequence identity with both mouse and rat IL15. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IL15 |
Protein | Interleukin-15 |
Uniprot ID | P40933 |
Function | Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. |
Tissue Specificity | Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus. |
Sub-cellular localization | Isoform IL15-S48AA: Secreted. |
Sequence Similarities | Belongs to the IL-15/IL-21 family. |
Aliases | IL 15 antibody|IL-15 antibody|IL15 antibody|IL15_HUMAN antibody|interleukin 15 antibody|Interleukin-15 antibody|Interleukin15 antibody|MGC9721 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IL15 antibody, ASA-B1006, Western blotting
All lanes: Anti IL15 (ASA-B1006) at 0.5ug/ml
WB: Recombinant Human IL15 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
All lanes: Anti IL15 (ASA-B1006) at 0.5ug/ml
WB: Recombinant Human IL15 Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
