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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human IKK gamma(97-114aa FQEARKLVERLGLEKLDL), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | IKBKG |
Protein | NF-kappa-B essential modulator(NEMO) |
Uniprot ID | Q9Y6K9 |
Function | Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. |
Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Contains 1 C2HC-type zinc finger. |
Aliases | IkB kinase associated protein 1 antibody|IkB kinase subunit gamma antibody|Inhibitor of nuclear factor kappa B kinase subunit gamma antibody|AMCBX1 antibody|FIP 3 antibody|FIP-3 antibody|FIP3 antibody|Fip3p antibody|I kappa B kinase gamma antibody|I-kappa-B kinase subunit gamma antibody|IkB kinase associated protein 1 antibody|IkB kinase gamma subunit antibody|IkB kinase subunit gamma antibody|IkB kinase-associated protein 1 antibody|IKBKG antibody|IKK-gamma antibody|IKKAP 1 antibody|IKKAP1 antibody|IKKG antibody|Incontinentia pigmenti antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells kinase gamma antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody|Inhibitor of nuclear factor kappa B kinase gamma subunit antibody|Inhibitor of nuclear factor kappa B kinase subunit gamma antibody|Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody|IP 1 antibody|IP 2 antibody|IP antibody|IP1 antibody|IP2 antibody|IPD2 antibody|NEMO antibody|NEMO_HUMAN antibody|NF kappa B essential modifier antibody|NF kappa B essential modulator antibody|NF kappaB essential modifier antibody|NF kappaB essential modulator antibody|NF-kappa-B essential modifier antibody|NF-kappa-B essential modulator antibody|NFkappaB essential modulator antibody|ZC2HC9 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IKBKG antibody, ASA-B0987, Western blotting
All lanes: Anti IKBKG (ASA-B0987) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti IKBKG (ASA-B0987) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
